Study Questions

1. An analog of cytidine is 5-azacytidine (5-azaC), which is taken up by cells and incorporated into their DNA. The presence of the azo group (as this N=N group is called when standing alone) at the 5' position makes it impossible to methylate this analog. There is a line of tissue culture cells (10T3-1/2) which, when exposed to 5-azaC, will differentiate in vitro into muscle cells. What explains this result?

ANSWER

2. What consequences for the development of Drosophila would arise from producing mutations in the gene for RNA polymerase III?

ANSWER

3. Suppose you could create a reporter gene to examine the function of various portions of the regulatory regions upstream of the endo16 gene in sea urchin embryos. Predict the outcome of the activity of a reporter in the embryo that is driven by the endo16 basal promoter and includes modules A, B, E, and F.

ANSWER

4. The oskar gene is one of several genes necessary for localization of nanos mRNA to the posterior pole of the Drosophila oocyte. What would be the effect of an oskar -/- mutation on development of the anteroposterior axis of Drosophila?

ANSWER

This site requires Internet Explorer 5.0 or higher / Mozilla-Compatible Browser,

This site also requires the Macromedia Flash player.
Get it here.

HOME | ABOUT THIS SITE | CONTACT US